Fig. 3. SUPT5H depletion suppresses the tumorigenesis and colony formation ability of breast cancer MDA-MB-231 cells. (A) Crystal violet assay was performed to determine the proliferation ability of breast cancer cells transfected with siRNA-SUPT5H (B) Cell viability of siRNA SUPT5H transfected cells was determined by CellTiter-Glo^(r) luminescent cell viability assay. (C) The Clonogenicity of SUPT5H depleted cells was determined by colony formation assay. Bars show the mean ± SD of at least triplicates. **p<0.01, ***p<0.001.